Critique 2 Essay Example | Topics and Well Written Essays - 750 words - 2

Critique 2 - Essay Example The theater also had a set stage where piano presentations were well orchestrated. Seok-Min having had the exposure and years of training on the piano. He got the opportunity to present his skills to the audience. The Sonata in C Major was a very slow and subtle piece written and composed by Joseph Haydn in the 17th and 18th century. There was also another piece B Minor composed by Franz Liszt and written in 1811 to 1886. The piece was long and well separated with hype and the mid and a slag finish. Although the piece was no interactive as then, performers had a fixed position as they keyed their way in the performance. In the recital hall, a number of musical pieces such as the piece by Claude Debussy, Etudes (1915). The piano presentation was primarily a senior grounds on which, I had the opportunity to get in touch with the other professional artist such as on ever. The piano presentation hall lacked proper organizations as the viewers were a section the performance was not as wel l orchestrated. The coordination and rhyme of the pattern lines as Seok-Min played were not in line with the other beats from the backup singers. The soprano presentation was very well planned but at the begin the lack time conscious meant that the piece had to be fast passed in order to appear like it was perfectly matched with the real thing. Seok-Min performance, on the other hand, was perfect as the support his musical pieces came through swiftly in the end. Their dressing also made the event look well organized as they marched to suit the theme they were reflecting in their musical pieces. The piano performers Seok-Min on the piano was also able to make his piece Sonata in B minor a success as his esteemed practice showed perfections as his confidence on the piano was visible although the theater. His mode and sample piece were different from the other one before him as it allowed for a chance in the treble. He incorporated major

Protien Essay Example | Topics and Well Written Essays - 1000 words

Protien - Essay Example Due to presence of a high degree of palindromic sequences changes were made in the 3rd codons for R and A, and the 1st codon for L based on the second priority codon preference. The changes reduced the chances of self-annealing and formation of secondary structures. The new gene sequence was thus: The next step was to choose the restriction enzymes for cutting out the complete gene after it has been cloned to the TOPO vector. NdeI, which cleaves the sequence CA/TATG was chosen for the 5’ end while BPu1102 which cuts GC/TGAGC was selected for the 3’ end. Both restriction enzymes do not have restriction sites in the chosen vector (but are present in the expression vector which will be used for protein expression later). GCGC nucleotides are added before and after the NdeI and BPu1102 sites respectively to act as primer initiation sites for PCR of the final gene sequence which is the following: 2. The PCR-based methods for gene synthesis normally require a DNA template, which is not available for designed peptides, for error-free amplification. To reduce error, nucleotide stretches of the optimized gene sequences are synthesized and ligated to complementary sequences followed by PCR amplification (Tsuchiya, Morioka, Shirai, Yoshida, & Inumaru, 2006) (Young & Dong, 2004). These procedures result in different gene fragments that have errors in the sequences. Further cloning, purification and sequencing for the desired gene sequence is expensive and time-consuming. In this study, the gene will be synthesized using circular assembly amplification, a new technique in gene synthesis that removes error sequences and increases the probability of getting accurate sequences (Bang & Church, 2008). Here, a mixture of short complementary oligonucleotides (~ 50bp), that are designed with overlaps to allow complementary coupling or annealing, generates circular DNA. This is followed by exonuclease treatment to remove